Hoechst 33342/propidium iodide pi staining
NettetAs correctly noted, PI stains nuclei, DNA, and therefore the cells must be permeable to the dye (have a damaged membrane). Maybe your drugs do not harm the cell membrane, but have a specific... NettetHoechst 33342 is a cell permeable fluorescent minor groove-binding probe for DNA, and through the living cell membrane. The Hoechst 33342 and DNA complex show light blue flourescent color. Propidium iodide (PI) has been widely used as a fluorescent stain for DNA in cells; it specificity binds double-stranded nucleic acids in apoptosis, necrosis …
Hoechst 33342/propidium iodide pi staining
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NettetThe effects of MTDH/AEG-1 siRNA on apoptosis and morphology in three cell lines (T24, RT4, and HT1376) were measured using Hoechst 33342 (Sigma-Aldrich Co., St Louis, … NettetFor live-cell staining, Hoechst 33342 dye is a popular cell-permeant nuclear counterstain. A special formulation of Hoechst 33342 dye, NucBlue Live ReadyProbes nucleic acid stain is supplied ready to use in a dropper bottle. Additional probes for nucleic acid staining Resources Fluorescence SpectraViewer Cell analysis learning center
Nettet28. feb. 2024 · Propidium iodide (PI; Beyotime Biotech, Nantong, China) and Hoechst 33342 (Beyotime Biotech) staining was also performed to quantify the necrotic cells. After laser irradiation, the cells were washed twice with PBS and stained with PI and Hoechst 33342 at 37°C for 20 minutes in the dark. Nettet9. mai 2024 · Propidium Iodide (PI)/Hoechst 33342 Staining To examine the membranolytic activities of peptides, the PI/Hoechst 33342 staining was performed in this study. The PI dye was used to stain damaged/dead cells, and Hoechst 33342 was used to specifically stain the nuclei of living cells [ 21 , 24 ].
NettetPropidium iodide (PI) and Hoechst 33342 double staining Open-i Gene therapy with HSV1-sr39TK/GCV exhibits a stronger therapeutic efficacy than HSV1-TK/GCV in rat … NettetDNA-binding dyes include propidium iodide (PI), 7-aminoactinomycin-D (7-AAD), Hoechst 33342, 33258 and S769121, TO-PRO-3, 4’6’-diamidino-2-phenylindole …
NettetHoechst 33342 is used for specifically staining the nuclei of living or fixed cells and tissues. This stain is commonly used in combination with 5-bromo-2'-deoxyuridine (BrdU) labeling to distinguish the compact chromatin of apoptotic nuclei, to identify replicating cells and to sort cells based on their DNA content.
NettetThis evidence suggests that Hoechst 33342 could be an unreliable marker for sperm-oocyte fusion in fertilization because of its capacity for passive movement from oocyte … senior center fergus falls mnNettetPropidium iodide (PI) is a red fluorescent stain that binds to DNA by intercalating between the bases. PI is unable to enter living cells, and so it stains cells that have … senior center garden city ksNettetThe protocol for DNA staining by Hoechst 33342 is as follows: Treat the cells with Hoechst 33342 for 10-60 minutes at 37°C. The concentration of Hoechst used must be pre-determined, as the optimal concentration … senior center federal wayNettetWe developed a rapid technique for preservation of Hoechst 33342/propidium iodide-stained cells, using ethanol as a fixative. Combined staining with these dyes makes … senior center falmouth maNettet9. mai 2024 · Propidium Iodide (PI)/Hoechst 33342 Staining To examine the membranolytic activities of peptides, the PI/Hoechst 33342 staining was performed in … senior center farragut tnhttp://www.cyto.purdue.edu/cdroms/cyto3/8/data/icrf/hpi.htm senior center fitchburg wiNettetInappropriate Hoechst-staining of cells: The assay depends on successful staining with Solution 15 (Hoechst-staining). In order to obtain this it is crucial to follow the protocol carefully. Thus, in step 2 it is mandatory to incubate at 37 °C for 15 minutes using a heating block. Do not use other Hoechst variants than Hoechst 33342/Solution 15. senior center forsyth mo