Phospho chk2 antibody
WebChk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven … WebJul 18, 2024 · CHK2 is phosphorylated at tyrosine 156 in nocodazole-arrested M phase cells To determine whether there is any M phase-specific CHK2 phosphorylation, ectopically expressed CHK2 was...
Phospho chk2 antibody
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WebThe oncogenic cytoplasmic p21 contributes to cancer aggressiveness and chemotherapeutic failure. However, the molecular mechanisms remain obscure. Here, we show for the first time that cytoplasmic p21 mediates 5-Fluorouracil (5FU) resistance by shuttling p-Chk2 out of the nucleus to protect the tumor cells from its pro-apoptotic …
WebFeb 4, 2024 · (A) Chk2 phosphorylation: Chk2 is phosphorylated by ATM kinase in response to DNA damage and is important in DNA damage induced checkpoint signaling. A549 cells were treated with vehicle (DMSO) or 50 µM etoposide for 3 hrs. After staining cells with Cellomics Chk2 activation kit, images were acquired and analyzed. WebSep 5, 2000 · ATM-dependent phosphorylation of Chk2 is seen only when cells are damaged by IR. Chk2 is phosphorylated in an ATM-independent manner in response to UV or HU ( 7, …
WebPVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human Phospho-FAK (Y397) Monoclonal Antibody (Catalog # MAB4528) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for Phospho-FAK (Y397) at approximately 150 kDa (as indicated). This experiment was conducted under … WebMar 25, 2024 · Flow cytometric analysis of phospho-Chk2 pThr68 on untreated (blue histogram) or UV-treated (green histogram) HeLa cells. Cells were harvested, fixed with formaldehyde, permeabilized with methanol, and incubated with a Phospho-Chk2 pThr68 polyclonal antibody (Product # PA5-17818) at a 1:800 dilution for 1 hour at room …
WebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt. Alias: Fold/Unfold
WebPhospho-Chk2 (Ser33/35) Antibody Cell Signaling Technology Polyclonal Antibody for studying Chk2 (Ser33/Ser35) phosphate. Cited in 26 publications. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Chk2 (Ser33/35) Antibody (CST #2665) is ready to ship. Go to your regional site? how many ml are in 3/4 teaspoonfulWebPVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-CDC2 (Y15) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF888), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-CDC2 (Y15) at approximately 34 kDa (as indicated). how many ml are in 5 tbspWebChk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each … how are you today chatgptWebMar 21, 2024 · CHEK2 (Checkpoint Kinase 2) is a Protein Coding gene. Diseases associated with CHEK2 include Prostate Cancer and Li-Fraumeni Syndrome 2 . Among its related pathways are Regulation of activated PAK … how many ml are in 4 klWebChk2 is a cell cycle checkpoint regulator and tumor suppressor. Along with Chk1, it plays a critical role in the cellular response to DNA damage, and is particularly important during … how many ml are in a fifthWebAnti-Chk2 (phospho T68) antibody See all Chk2 primary antibodies Description Rabbit polyclonal to Chk2 (phospho T68) Host species Rabbit Tested applications Suitable for: … how many ml are in 8 0zWebImmunocytochemistry/ Immunofluorescence: Chk2 [p Thr68] Antibody [NBP3-13076] - Chk2 (phospho Thr68) antibody detects Chk2 (phospho Thr68) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa ...read more how are you tlumacz